S1). The surrounding substrate was passivated to constrain outgrowth of the P hPSC colonies. Cells in P hPSC colonies could maintain pluripotency and show similar gene and protein expression levels compared to conventionally cultured hPSCs cultured in mTeSRTM1 maintenance medium (Supplementary Fig.
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For quality filtering, sequences that had average quality scores of (Q over a 50 bp sliding window, lacked an accurate primer sequence, contained ambiguous base call or possessed more than eight homopolymers were excluded from analyses. Sequences were then aligned against the SILVA alignment template (Pruesse et al., 2007). The high quality sequences were pre clustered as previously described (Huse et al., 2010) to further reduce any potential influence of sequencing errors.