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Core interaction is dispensable for ERK2 bindingActivation of ERK MAP kinase has been extensively used as a primary readout of arr dependent signalling downstream of GPCRs23,24,25,26. Arrs directly interact with ERK2 as well as upstream kinases of ERK cascade (c Raf1 and MEK1) and it is proposed that arrs act as scaffolds to bring the components of ERK cascade together27,28,29,30. We first measured the interaction of purified arr1 with inactive and active ERK2 in the absence or presence of a phosphopeptide corresponding to the carboxyl terminus of the vasopressin receptor (V2Rpp).
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