A fat exclusion lunch containing 3.1 g dietary fat and comprising vegetarian pasta, bread roll and orange juice was served immediately following the 6 h blood sample. A fat exclusion snack containing 0.7 g dietary fat and comprising fruit cake and apple juice was served mid afternoon, 8 h postbreakfast. A fat exclusion dinner containing 1.3 g dietary fat and comprising vegetarian risotto, raspberry desert and a soft beverage was served following the 10 h blood sample.
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To study the effects of each of these mutations on the phosphorylation profile of sMyBP C, we used human biopsies of the abductor hallucis (AH) and gastrocnemius muscles carrying the Y856H and W236R mutations, respectively; AH and gastrocnemius muscles that did not contain the DA 1 mutations served as controls. We generated protein lysates from the DA 1 and control muscles and analyzed them by conventional or phosphate affinity gel electrophoresis and subsequent immunoblotting with the pan sMyBP C and the four phospho specific antibodies. As a result of the high homology of the four phospho sites between mouse and human (Fig.